Figure 5.

The AdipoR agonist, AdipoRon, decreases myometrial contractility for both spontaneous and oxytocin-induced contractions in E15 uterine strips and activates AMPK. A) Contractile tension as AUC is shown for spontaneously contracting (left; n = 13) and oxytocin-stimulated (n = 12) myometrial strips treated with increasing cumulative concentrations of AdipoRon (0.1–45 µM) or the equivalent volume of vehicle. Representative tracings and analysis for peak height and cyclic frequency are shown in Supplemental Fig. S6. **P < 0.01, ***P < 0.001, ****P < 0.0001. B) Representative blots show duplicate preparations of AdipoRon-treated myometrial strips from organ bath, expressing total AMPK (AMPK) and AMPK phosphorylated at Thr172 (P-thr172-AMPK). Strips were treated with 45 µM AdipoRon or equivalent volume of vehicle. Spontaneous contractions (n = 9–12) and oxytocin-induced contractions (n = 8). IC₅₀, half maximal inhibition concentration. *P < 0.05.