Serum proinflammatory cytokine expression is attenuated by CDC administration. A–C) ELISA measured the expression of circulating serum cytokines, including IL-1β (A), IL-6 (B), and TNF-α (C) in KP and KP + CDC mice (n = 5/group). D–F) The expression of lung proinflammatory genes IL-6 (D), TNF-α (E), and IL-1β (F) were measured by real-time PCR following in KP and KP + CDC mice (n = 5/group). G, H) The expression of lung components of inflammasomes, including NLRP-3 (G) and procaspase-1 (H), was measured by real-time PCR in KP and KP + CDC mice (n = 5/group). I) The expression of the whole lung lysate protein NLRP-3 and caspase-1 was measured by Western blot in KP and KP + CDC mice (n = 3/group). Statistical analysis was performed for each time point. Samples were analyzed using a 2-tailed, unpaired Student’s t test with Welch’s correction. *P < 0.05 CDC vs.
KP; #P < 0.05 KP
vs.
KP + CDC.