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. 2019 Oct 26;33(12):14221–14233. doi: 10.1096/fj.201901247R

Figure 4.

Figure 4

Substaging meiotic prophase I oocytes from e13.5 cultured ovaries using 15 and 50 nM of siRNA. A) Immunostaining of Sycp3 (red) and Sycp1 (green) to assess axial element and transverse filament formation, respectively. Representative merged images displayed. Scale bars, 10 μm, are consistent across 15 and 50 nM culture images. B) Relative proportions of pachytene, early diplotene, and late-diplotene and dictyate oocytes from d 5 cultured ovaries following 15 nM siRNA treatment. Bar graphs display mean percentages from 3 repeats, and a total of 125 scramble siRNA, and 166 Dazl siRNA–cultured oocytes. C) Relative proportions of pachytene, early diplotene, and late-diplotene and dictyate oocytes from d 5 cultured ovaries following 50 nM siRNA treatment. Bar graphs display mean percentages from 3 repeats, and a total of 291 scramble siRNA, and 287 Dazl siRNA–cultured oocytes. Con, control; KD, knockdown. Error bars indicate means ± se.