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. 2019 Oct 26;33(12):14221–14233. doi: 10.1096/fj.201901247R

Figure 7.

Figure 7

DAZL binds the 3′UTR of TEX14 and stimulates translation. A) Graph displays counts per million mapped reads of TEX14 taken from DAZL RNA-immunoprecipitation and sequencing experiment described in Rosario et al. (24). Circles depict data from individual experiments. Error bars indicate means ± se. B) 3′UTR-luciferase reporter activity in the presence of empty vector control (magenta bar), DAZL (blue bar), or R115G DAZL mutant (green bar). Firefly luciferase signals were normalized to Renilla luciferase signals. Error bars indicate means ± se; n = 4. P = 0.027, Dunns multiple comparison test. C) TEX14 3′UTR-luciferase mRNA expression in the presence or absence of DAZL. Expression was analyzed 48 h after transfection. Firefly luciferase mRNA expression was normalized to Renilla luciferase mRNA expression. Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Error bars indicate means ± se; n = 4 for each group. hLuc, humanised firefly luciferase; rLuc, Renilla luciferase.