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. 2019 Oct 26;33(12):14147–14158. doi: 10.1096/fj.201900786R

Figure 1.

Figure 1

EC-specific Glrx overexpression impaired EC migration in vivo. A) Protein extracts from lungs in EC-Glrx TG mice with or without DOX were blotted and probed with antibodies directed against FLAG. B) Lung proteins from the control and EC-Glrx TG mice without DOX were blotted with the anti-Glrx antibody; the upper band indicates exogenous FLAG-Glrx, whereas the lower band indicates endogenous Glrx. C) FLAG-Glrx (red) colocalizes with the EC marker isolectin B4 (green) in cross sections of the gastrocnemius muscle. D) EC migration in vivo assay. Hemoglobin content in Matrigel plugs implanted subcutaneously in EC-Glrx TG (EC-TG) and control mice for 2 wk with or without VEGF (n = 8). *P < 0.05. The photos show VEGF-containing Matrigel plugs (scale bars, 5 mm) and immunohistochemistry for CD31 (red arrows; scale bars, 100 μm) in the plugs from control (C) and EC-Glrx TG mice.