TABLE 4.

Western blot analysis of P-gp and hmPGES-1 protein expression levels in isolated brain capillaries exposed to glutamate and BI1029539

Exposure	P-gp	hmPGES-1
Control	100 ± 4.8	100 ± 3.1
100 µM glutamate	174.9 ± 6.6*	149.5 ± 5.5*
1 nM BI1029539	103.3 ± 5.7	131.4 ± 4.7*
10 nM BI1029539	103.9 ± 5.1	97.4 ± 2.3
100 nM BI1029539	100.2 ± 5.3	100.2 ± 3.4

Data were normalized to β-actin levels; values are given as the percentage of control ± sem (n = 3). See Fig. 6A.

^*P < 0.01 for each endpoint compared with control (Dunnett’s many-to-one t test).