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. 2019 Nov 25;33(12):13617–13631. doi: 10.1096/fj.201901123R

Figure 1.

Figure 1

APN−/− mice show up-regulated expression of endothelial adhesion markers and are related to mitochondrial dysfunctional in lung endothelium. A, B) Western blot analysis for ICAM-1, VCAM-1, and E-selectin (Esel.) in the whole lung tissues and in freshly isolated lung ECs in APN−/− and WT control mice at bsl. GAPDH is used as loading control. C–E) Western blot analysis for PGC-1α, NRF-1, TFAM, Sirt3, and Sirt1 expression in whole lung tissues and in freshly isolated lung ECs in APN−/− and WT control mice at bsl (GAPDH and β-actin are used as loading controls). F) NAD+/NADH ratio in the lungs of APN−/− and WT control mice at bsl. G) Expression of mitochondrial oxidative phosphorylation complexes (I, II, III, IV, and V) in the lungs of APN−/− and WT control mice at bsl. Ns, not significant. Densitometry analysis is shown on the right. Data are expressed as means ± se; n = 6 in each group. *P < 0.05, **P < 0.01, ***P < 0.001.