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. 2019 Nov 25;33(12):13617–13631. doi: 10.1096/fj.201901123R

Figure 4.

Figure 4

Restoration of mitochondrial biogenesis reverses lung endothelial activation in APN-deficient mice. A, B) Transcript (PGC-1α, NRF-1, Sirt3) and protein expression (PGC-1α, NRF-1, Sirt1, and Sirt3) in the lungs of APN−/− mice treated with and without PQQ; n = 6 in each group. C) Expression of mitochondrial oxidative phosphorylation complexes (I, II, III, IV, and V) in the lungs of APN−/− mice treated with and without PQQ; n = 6 in each group. D) NAD+/NADH ratio in the lungs of APN−/− mice treated with and without PQQ; n = 8 in each group. E) Western blot analysis for p62 protein in the lungs of APN−/− mice treated with and without PQQ; n = 6 in each group. F, G) Western blot analysis for Pink1 and Parkin in cytosol and mitochondrial fractions isolated from lungs of APN−/− mice treated with and without PQQ; n = 6 in each group. H) Western blot analysis for NLRP3, activated caspase-1, and cleaved IL-1β in whole lung tissues; n = 6 in each group. *P < 0.05, **P < 0.01. I) Western blot analysis for VCAM-1 and E-selectin in whole lung tissues; n = 6 in each group. Ns, not significant. Densitometry analyses are shown on the right. Data are expressed as means ± se. *P < 0.05, **P < 0.01, ***P < 0.001.