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. 2019 Nov 25;33(12):13617–13631. doi: 10.1096/fj.201901123R

Figure 5.

Figure 5

Up-regulation of mitochondrial biogenesis and function in lung ECs mitigates endothelial activation and barrier function in response to LPS. A) Western blot analysis for PGC-1α, NRF-1, TFAM, and Sirt3 in lung ECs pretreated with PQQ and stimulated with LPS (1 μg/ml for 24 h). B) Confocal microscopy analysis of mitochondria morphology in lung ECs stained with MitoTracker Green. Quantification of MitoTracker Green is shown on the right. C) Decreased mt-ROS production (MitoSox) as shown in red and merged with nuclear stain DAPI in blue in lung ECs treated with PQQ and stimulated with LPS. D–F) Mitochondrial membrane potential, NAD+/NADH, and ATP content in lung ECs pretreated with PQQ and stimulated with LPS. G) Western blot analysis for ICAM-1, VCAM-1, and E-selectin in lung ECs treated with PQQ and stimulated with LPS. H) Endothelial permeability measured by FITC-dextran permeability to lung ECs treated with PQQ and stimulated with LPS. MFI, mean fluorescence intensity. Densitometry analyses are shown on the right; n = 4 in each group. *P < 0.05, **P < 0.01, ***P < 0.001.