TABLE 1.
Concentrations of EC and IC inorganic ions in heart, liver, and red blood cells
| Perfused beating heart |
Liver |
Red blood cell,
control |
||||
|---|---|---|---|---|---|---|
| Ion/location |
Glucose |
G + insulin |
G + ketones |
Control |
+ Na acetate |
|
| Ca2+ EC | 1.1 | 1.1 | 1.1 | 1.1 | 1.07 | 1.07 |
| Ca2+ IC | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
| Na+ EC | 144 | 144 | 149 | 146 ± 1 | 170 ± 2 | 144 |
| Na+ IC | 6.5 ± 5.2 | 5.6 ± 1.8 | 5.1 ± 0.8 | 12.3 ± 1.2 | 10.7 ± 1.5 | 8.8 ± 6.3 |
| Mg2+ EC | 0.5 | 0.5 | 0.5 | 0.52 ± 0.0 | 0.51 ± 0.02 | 0.52 ± 0.02 |
| Mg2+ IC | 1.2 ± 0.03 | 0.72 ± 0.3 | 0.74 ± 0.1 | 0.43 ± 0.0 | 0.97 ± 0.01 | 0.5 |
| pH external | 7.42 | 7.39 | 7.4 | 7.37 ± 0.03 | 7.52 ± 0.0 | 7.40 |
| pH internal | 7.06 ± 0.01 | 7.05 ± 0.2 | 7.04 ± 0.03 | 7.12 ± 0.07 | 7.12 ± 0.1 | 7.29 ± 0.0 |
| K+ EC | 5.9 | 5.9 | 5.9 | 5.22 ± 0.1 | 3.9 ± 0.3 | 5.16 ± 0.3 |
| K+ IC | 142 ± 3 | 147 ± 5 | 141 ± 6 | 175 ± 1 | 229 ± 2 | 153 ± 5 |
| Cl− EC | 127 | 127 | 127 | 103 ± 1 | 95.4 ± 1 | 108 ± 1 |
| Cl− IC | 47.9 ± 3.6 | 29.4 ± 5.5 | 36.1 ± 11 | 36.7 ± 2.3 | 30.8 ± 2.2 | 83.4 ± 2.9 |
| HCO3− EC | 25 | 25 | 25 | 26.7 ± 0.5 | 36.5 ± 1.4 | 26.7 ± 0.5 |
| HCO3− IC | 14.3 ± 0.2 | 14.4 ± 0.5 | 12 ± 0.4 | 15.1 ± 1.1 | 19.0 ± 1.6 | 22 ± 0.7 |
| H2PO4− EC | 0.18 | 0.19 | 0.19 | 0.22 ± 0.01 | 0.11 ± 0.01 | 0.39 ± 0.01 |
| H2PO4− IC | 2.43 ± 0.2 | 1.8 ± 0.2 | 2.9 ± 0.2 | 0.356 ± 0.08 | 0.48 ± 0.01 | 0.27 ± 0.02 |
| HPO42− EC | 0.91 | 0.90 | 0.91 | 0.95 ± 0.03 | 0.67 ± 0.04 | 1.9 ± 0.1 |
| HPO42− IC | 5.4 ± 0.4 | 4.03 ± 0.3 | 6.26 ± 0.4 | 0.91 ± 0.2 | 1.55 ± 0.45 | 1.0 ± 0.1 |
The values are given as means ± SEM in units of micromoles per milliliter. H2O free intracellular [Ca2+] was assigned as 0.2 μM in all tissues (11). EC, extracellular; IC, intracellular.