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. 2019 Nov 5;33(12):14748–14759. doi: 10.1096/fj.201901279R

Figure 2.

Figure 2

Generation of Pradc1 KO mice. A) Schematic showing the gene-targeting strategy used to generate Pradc1 KO mice. B) PCR genotyping results showing the successful generation of WT+/+ and homozygous KO−/− mice. C) The absence of Pradc1 mRNA in mouse tissues of KO mice was confirmed by real-time PCR. eWAT, epididymal white adipose tissue; loxP, cleavage sequence for Cre recombinase; Neor, neomycin resistant; pA, polyadenylation signal.