Figure 3.

Reduced adiposity and enhanced physical activity and energy expenditure in Pradc1 KO female mice fed an HFD. A) Body weight gain over time in WT and Pradc1 KO female mice fed an HFD. B) Representative image of WT and KO female mice at 25 wk old. C) Whole-body fat mass of WT and KO female mice as determined by Echo-MRI. D) Percent fat mass relative to body weight. E) Whole-body lean mass of WT and KO female mice as determined by nuclear magnetic resonance. F) Percent lean mass relative to body weight. G, H) Food intake during the light (G) and dark (H) cycle. I, J) Rate of oxygen consumption (Vo₂) as a measure of metabolic rate in WT and KO female mice during the light (I) and dark (J) cycle. K) Total physical activity as measured by beam breaks in WT and KO female mice during the light and dark cycle. L) Energy expenditure of WT and KO female mice during the light and dark cycle. Female mice: WT, n = 9; KO, n = 13. Mice were at 25–26 wk of age when indirect calorimetry analyses were performed. Data are expressed as means ± sem. *P < 0.05, **P < 0.01.