TABLE 2.
Real-time PCR analysis of the expression of metabolic, inflammatory, and oxidative stress genes in visceral and subcutaneous fat depots of WT and Pradc1 KO female mice fed an HFD
| Visceral gWAT |
s.c. iWAT |
||||||
|---|---|---|---|---|---|---|---|
| Gene | Gene name | WT | KO | P | WT | KO | P |
| Fatty acid synthesis | N = 8 | N = 8 | N = 8 | N = 8 | |||
| Fasn | Fatty acid synthase | 1.00 ± 0.26 | 1.28 ± 0.41 | 0.574 | 1.00 ± 0.31 | 0.58 ± 0.19 | 0.3044 |
| Acc1 | Acetyl-CoA carboxylase | 1.00 ± 0.22 | 0.74 ± 0.16 | 0.385 | 1.00 ± 0.16 | 0.89 ± 0.32 | 0.768 |
| Scd1 | Stearoyl-CoA desaturase-1 | 1.00 ± 0.16 | 0.92 ± 0.26 | 0.816 | 1.00 ± 0.09 | 0.62 ± 0.10* | 0.022 |
| Srebp-1c | Sterol regulatory element-binding transcription factor 1C | 1.00 ± 0.11 | 1.01 ± 0.22 | 0.946 | 1.00 ± 0.34 | 0.54 ± 0.16 | 0.291 |
| Ppar-γ | Peroxisome proliferator activated receptor γ | 1.00 ± 0.16 | 0.74 ± 0.13 | 0.247 | 1.00 ± 0.20 | 0.55 ± 0.19 | 0.146 |
| Triglyceride synthesis | |||||||
| Gpat1 | Glycerol-3-phosphate acyltransferase 1 | 1.00 ± 0.11 | 1.08 ± 0.12 | 0.635 | 1.04 ± 0.08 | 0.82 ± 0.18 | 0.545 |
| Gpat2 | Glycerol-3-phosphate acyltransferase 2 | 1.00 ± 0.20 | 0.68 ± 0.16 | 0.256 | N/A | N/A | |
| Agpat1 | 1-Acyl-glycerol-3-phosphate acyltransferase 1 | 1.00 ± 0.08 | 0.89 ± 0.14 | 0.534 | 1.00 ± 0.08 | 1.47 ± 0.38 | 0.260 |
| Agpat2 | 1-Acyl-glycerol-3-phosphate acyltransferase 2 | 1.00 ± 0.20 | 1.13 ± 0.26 | 0.698 | 1.00 ± 0.22 | 0.41 ± 0.05* | 0.046 |
| Dgat1 | Diacylglycerol O-Acyltransferase 1 | 1.00 ± 0.08 | 0.89 ± 0.16 | 0.586 | 1.00 ± 0.15 | 0.71 ± 0.24 | 0.337 |
| Dgat2 | Diacylglycerol O-Acyltransferase 2 | 1.00 ± 0.12 | 1.16 ± 0.26 | 0.560 | 1.00 ± 0.19 | 0.42 ± 0.07* | 0.032 |
| Lipolysis | |||||||
| Atgl | Adipose triglyceride lipase | 1.00 ± 0.17 | 0.91 ± 0.09 | 0.663 | 1.00 ± 0.14 | 0.90 ± 0.31 | 0.776 |
| Hsl | Hormone-sensitive lipase | 1.00 ± 0.17 | 0.78 ± 0.16 | 0.389 | 1.00 ± 0.13 | 0.72 ± 0.22 | 0.317 |
| Fat oxidation | |||||||
| Ppar-α | Peroxisome proliferator activated receptor α | 1.00 ± 0.26 | 0.87 ± 0.15 | 0.708 | 1.00 ± 0.16 | 0.84 ± 0.31 | 0.672 |
| Cpt-1a | Carnitine palmitoyltransferase 1A | 1.00 ± 0.12 | 0.75 ± 0.14 | 0.205 | 1.00 ± 0.16 | 0.59 ± 0.17 | 0.112 |
| Acox1 | Acyl-CoA oxidase 1 | 1.00 ± 0.14 | 0.90 ± 0.11 | 0.600 | 1.00 ± 0.19 | 0.72 ± 0.31 | 0.479 |
| Lcad | Long chain acyl-CoA dehydrogenase | 1.00 ± 0.08 | 0.69 ± 0.12 | 0.061 | 1.00 ± 0.12 | 0.88 ± 0.33 | 0.757 |
| Mcad | Medium chain acyl-CoA dehydrogenase | 1.00 ± 0.16 | 0.78 ± 0.19 | 0.415 | 1.00 ± 0.13 | 0.89 ± 0.30 | 0.766 |
| Hadha | Trifunctional enzyme subunit α | 1.00 ± 0.11 | 0.58 ± 0.07* | 0.010 | 1.00 ± 0.13 | 0.82 ± 0.27 | 0.562 |
| Inflammation | |||||||
| Tnf-α | Tumor necrosis factor α | 1.00 ± 0.11 | 0.58 ± 0.09* | 0.013 | 1.00 ± 0.16 | 0.51 ± 0.05* | 0.021 |
| IL-1β | Interleukin 1-β | 1.00 ± 0.15 | 1.38 ± 0.40 | 0.397 | 1.00 ± 0.15 | 3.34 ± 1.11 | 0.073 |
| IL-6 | Interleukin 6 | 1.00 ± 0.17 | 0.78 ± 0.13 | 0.352 | 1.00 ± 0.32 | 0.87 ± 0.23 | 0.767 |
| Mcp-1 | Monocyte chemoattractant protein 1 | 1.00 ± 0.15 | 1.11 ± 0.28 | 0.719 | 1.00 ± 0.12 | 0.55 ± 0.05** | 0.005 |
| Ccr2 | C-C chemokine receptor type 2 | 1.00 ± 0.12 | 1.16 ± 0.27 | 0.598 | 1.00 ± 0.13 | 1.00 ± 0.20 | 0.980 |
| Ccl3 | C-C Motif chemokine ligand 3 | 1.00 ± 0.12 | 0.65 ± 0.12 | 0.072 | 1.00 ± 0.12 | 0.37 ± 0.03*** | 0.001 |
| Ccl4 | C-C Motif chemokine ligand 4 | 1.00 ± 0.10 | 0.92 ± 0.14 | 0.687 | 1.00 ± 0.16 | 0.51 ± 0.05* | 0.021 |
| Nos2 | Nitric oxide synthase 2 | 1.00 ± 0.23 | 1.19 ± 0.33 | 0.647 | 1.00 ± 0.15 | 0.81 ± 0.26 | 0.550 |
| F4/80 | EGF-like module-containing mucin-like hormone receptor-like 1 | 1.00 ± 0.11 | 0.62 ± 0.08* | 0.025 | 1.00 ± 0.09 | 0.55 ± 0.07** | 0.002 |
| Mgl2 | Macrophage galactose N-acetyl-galactosamine-specific lectin 2 | 1.00 ± 0.19 | 1.20 ± 0.27 | 0.554 | 1.00 ± 0.13 | 1.37 ± 0.33 | 0.307 |
| Cd206 | Cluster of differentiation 206 | 1.00 ± 0.17 | 1.57 ± 0.35 | 0.171 | 1.00 ± 0.14 | 0.62 ± 0.11 | 0.055 |
| IL-10 | Interleukin 10 | 1.00 ± 0.07 | 0.63 ± 0.11* | 0.019 | 1.00 ± 0.11 | 1.18 ± 0.24 | 0.491 |
| Arg1 | Arginase 1 | 1.00 ± 0.22 | 0.82 ± 0.17 | 0.556 | 1.00 ± 0.13 | 0.85 ± 0.24 | 0.646 |
| Cd68 | Cluster of differentiation 68 | 1.00 ± 0.12 | 0.66 ± 0.13 | 0.100 | 1.00 ± 0.16 | 0.46 ± 0.11* | 0.016 |
| Retnl | Resistin-like | 1.00 ± 0.44 | 1.70 ± 0.46 | 0.295 | 1.00 ± 0.13 | 1.21 ± 0.41 | 0.641 |
| Fibrosis | |||||||
| Tgf-β | Tranforming growth factor β | 1.00 ± 0.13 | 0.44 ± 0.08** | 0.004 | 1.00 ± 0.14 | 0.58 ± 0.16 | 0.081 |
| Col3a1 | Collagen type III alpha 1 chain | 1.00 ± 0.19 | 0.80 ± 0.14 | 0.4414 | 1.00 ± 0.16 | 0.60 ± 0.17 | 0.112 |
| Col6a1 | Collagen type VI alpha 1 chain | 1.00 ± 0.10 | 0.99 ± 0.11 | 0.988 | 1.00 ± 0.10 | 0.78 ± 0.20 | 0.391 |
| Hif-1α | Hypoxia-inducible factor 1 α | 1.00 ± 0.08 | 1.04 ± 0.11 | 0.763 | 1.00 ± 0.24 | 0.81 ± 0.27 | 0.617 |
| ER stress | |||||||
| Xbp-1s | X-box binding protein 1 (spliced) | 1.00 ± 0.08 | 0.83 ± 0.13 | 0.329 | 1.00 ± 0.13 | 0.74 ± 0.13 | 0.201 |
| Xbp-1t | X-box binding Protein 1 (unspliced) | 1.00 ± 0.07 | 0.81 ± 0.08 | 0.115 | 1.00 ± 0.07 | 0.90 ± 0.07 | 0.393 |
| Chop | C/EBP homologous protein | 1.00 ± 0.05 | 0.87 ± 0.07 | 0.212 | 1.00 ± 0.18 | 0.59 ± 0.13 | 0.098 |
| Atf4 | Activating transcription factor 4 | 1.00 ± 0.06 | 0.78 ± 0.06* | 0.037 | 1.00 ± 0.06 | 0.81 ± 0.10 | 0.167 |
| Oxidative stress | |||||||
| Sod1 | Superoxide dismutase 1 | 1.00 ± 0.14 | 0.97 ± 0.12 | 0.892 | 1.00 ± 0.08 | 0.94 ± 0.11 | 0.740 |
| Sod2 | Superoxide dismutase 2 | 1.00 ± 0.10 | 1.06 ± 0.12 | 0.713 | 1.00 ± 0.15 | 0.80 ± 0.18 | 0.468 |
| Cox2 | Cyclooxygenase 2 | 1.00 ± 0.21 | 1.05 ± 0.24 | 0.860 | 1.00 ± 0.18 | 0.86 ± 0.22 | 0.636 |
| Nox4 | NADPH oxidase 4 | 1.00 ± 0.12 | 0.94 ± 0.10 | 0.762 | 1.00 ± 0.11 | 0.87 ± 0.16 | 0.539 |
| Nqo1 | NAD(P)H quinone dehydrogenase 1 | 1.00 ± 0.21 | 0.50 ± 0.13 | 0.077 | 1.00 ± 0.10 | 0.51 ± 0.05** | 0.001 |
*P < 0.05, **P < 0.01, ***P < 0.001. N/A, not applicable.