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. 2019 Nov 2;33(12):14528–14541. doi: 10.1096/fj.201901570R

Figure 3.

Figure 3

The effect of volatile anesthetics on LPS-mediated NF-kB activation and their binding to TLR4-MD-2 complex. The effect of anesthetics on NF-kB activation by LPS (1 µg/ml) was examined by incubating Raw-blue cells (A) and THP-1 blue cells (B) with isoflurane (1%) or sevoflurane (2%) for 6 h. MD-2 was added to Raw-blue cells. Data were shown as means ± sd of quadruplicates. Statistical analysis was performed using 1-way ANOVA with Bonferroni post hoc analysis. ***P < 0.001. C) The effect of isoflurane and sevoflurane on NF-kB activation by LPS (1 µg/ml) was tested in THP-1 blue cells at a range of concentrations. D) TLR4–MD-2 complex protein was photolabeled with azi-isoflurane or azi-sevoflurane and subjected to MS analysis. Adducted residues were shown. Specificity of interaction was confirmed by competing with isoflurane or sevoflurane. More detailed information of MS analysis is shown in Supplemental Fig. S1B, C. OD, optical density.