Figure 5.

Rapid Ser^408−409 phosphorylation of GABA_A receptor β₃ subunit. A) Whole-cell fractions of dissected dorsal CA1 were analyzed. Molecular masses of standards are indicated on the right. B) Total GABA_A receptor β₃ subunit was normalized to total β-tubulin, showing an increase in the subunit 30 min after the training. C) The phosphorylation-specific signals of Ser^408−409 were normalized to the total GABA_A receptor β₃ subunit. IA training increased Ser^408−409 phosphorylation within 1 min after the training (0 min). *P < 0.05, **P < 0.01 vs. untrained; n = 9 in all groups. Error bars indicate ± sem. ▼ indicates the timing of training.