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. 2019 Nov 25;33(12):13503–13514. doi: 10.1096/fj.201901120R

Figure 1.

Figure 1

Cytc purified from porcine brain tissue is phosphorylated at S47 under basal conditions. A) Purified porcine brain Cytc is Ser phosphorylated. Top, Coomassie gel; bottom, anti-phosphoserine (pSer) Western blot (WB). B) The reduced absorption spectrum of porcine brain Cytc (13 µM) showing its characteristic α (550–558), β (521–527), and γ (415–423) peaks. C) nano–liquid chromatography electrospray ionization tandem mass spectrometric spectrum of KTGQAPGFpSYTDANK indicates that porcine brain Cytc is phosphorylated on S47. The phosphorylation site was unambiguously revealed by fragment ions y6 and y7. Abs, absorbance.