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. 2019 Nov 25;33(12):14194–14203. doi: 10.1096/fj.201901074R

Figure 1.

Figure 1

Construction of ACSL6 KO mice. A) ACSL6 KO mice were produced by CRISPR/Cas9 using sgRNAs targeting introns 10 and 11. A 1-kbp-deletion mutant was produced that lacked 2 copies of exon 11. Using 2 forward (Fw) primers (located 3′ and 5′ of the deletion site in intron 10) and a reverse (Rv) primer (located 3′ of the deletion site in intron 11), we confirmed the deletion of ACSL6 exon 11. B) Genotyping results for ACSL6 WT, HZ, and KO mice. PCR of the WT allele yielded a PCR product from Fw primer 2. PCR of the KO allele amplified a product from Fw primer 1.