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. 2019 Nov 25;33(12):14194–14203. doi: 10.1096/fj.201901074R

Figure 2.

Figure 2

ACSL6 produces DHA phospholipid in spermatids. A) ACSL6 expression was determined by RT-PCR from total RNA extracted from different tissues. B) Percentage of total PC in ACSL6 HZ and KO testes. Only phospholipids present in excess of 1% are shown. C) Abundance of major phospholipid species in ACSL6 HZ and KO testes. Data are presented as means ± sem (n = 3). D) Enzymatic activity of WT and KO testes. Data are presented as means ± sem (n = 5). E, F) mRNA expression of ACSL6 (E) and LPAAT3 (F) in isolated spermatids compared with that of the whole testis. mRNA levels were normalized to GAPDH. G) Percentage of AA (20:4)-, DHA (22:6)-, and DPA (22:5)-containing PC in isolated spermatids. SG, spermatogonia. Data are presented as means ± sem (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired Student’s t test).