Fig. 1.

Experimental design. a Experimental design for mice experiments. SAH was induced with surgery at D0. At 12 h (nSHAM = nSAH = 8), the mice were sacrificed, and brain and blood were prepared for flow cytometry analyses. At D1 blood was collected by saphenous bleeding for flow cytometry (nSHAM = 35, nSAH = 34) or the mice were sacrificed, and brain and blood were prepared for flow cytometry analyses (nSHAM = 16, nSAH = 18). At D2, blood was collected by saphenous bleeding for flow cytometry (nSHAM = nSAH = 19) or the mice were sacrificed, and brain and blood were prepared for flow cytometry analyses (nSHAM = 16, nSAH = 15). At D7, the phenotypic tests were performed (nSHAM = nSAH = 19) and blood and the brains were conserved, and paraffin embedded for immunofluorescence essays (nSHAM = nSAH = 11) or brains were prepared for flow cytometry (nSHAM = nSAH = 8). b Experimental design for human immunomonitoring. At D0, SAH patients were recruited within the first 48 h. At D0, D1, D2, D5, and D10, blood was collected and centrifuged for banking plasma. Plasma were used for cytokine analyses by MSD technology