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. 2019 Dec 4;10:369. doi: 10.1186/s13287-019-1443-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Confirmation of ADSCs differentiation. TGF-β1 level (pg/ml) (a), total protein (μg/ml) (b), TGF-β1 levels per total protein (pg/mg) (c) secreted by ADSC-SS after starvation for 24 h on day 7, 14 and 21 of observation. ADSC-SS in PRP and negative control are gradually increased on day 14 and 21 while ADSC-SS in positive control is stable. The TGF-β1 level which secreted by ADSC-SS PRP group shows significantly different to both of controls on day 7, 14, and 21 of observation; GAG level is dramatically increased in the ADSC-SS PRP and positive control groups and significantly different compared to that in the ADSC-SS negative control on day 21 (d); mineralization of ADSC-SS in PRP and control groups are stable in low level for 21 day of observation (e); and cell-surface protein expression in ADSC-SS group on day 21 passage 4 compared to that in monolayer ADSCs passage 3, both of ADSC-SS in PRP and positive control are significantly decreased their specific stem cell surface-protein markers (CD73, CD90, CD105) compared to that the ADSC-SS-PS (monolayer) (f). (*p < 0.05 indicates statistical significance)