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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Dec 11;127:125–133. doi: 10.1016/j.yjmcc.2018.12.004

Figure 1. Q73 expression in H9C2 cells induces mitochondrial damage mediated through Drp1 hyperactivation.

Figure 1.

A. Expression of polyglutamine repeats (Q23 or Q73) in H9C2 cardiomyocytes. B. H9C2 cardiomyocytes were transfected with either Q23 or Q73 repeats and after 48 hours were treated with P110 (1μM once) or Veh, for an additional 24 h in serum free- galactose medium and stained with anti-TOM20 (a marker of mitochondria, 1:500 dilution). Scale bar: 20 μm. 5 independent experiments. C. Mitochondrial aspect, roundness and form factor were determined. D. Levels of mitochondrial Drp1 were determined by immunoblotting, quantified and presented as ratio vs VDAC, loading control, treated as in (B). (n=6/group). Data were evaluated by one-way ANOVA and Holm-Sidak's multiple comparisons test for multiple testing between each treatment group. **** p-value <0.0001; *** p-value <0.001; ** p-value <0.01. All graphs represent mean ± s.d.

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