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. 2019 Dec 4;10:370. doi: 10.1186/s13287-019-1476-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — AFSC inhibit apoptosis in kidneys with ARF. a Representative immunoblots showing the expression of apoptotic proteins, viz, p53, Bax, Bcl-2, PUMA, cleaved caspase-3, and cleaved caspase-9, in kidney tissues of healthy control, saline-treated, and AFSC-treated groups on day 8 and day 12 after cisplatin injection. b Bar diagrams showing semi-quantitative densitometric determination of ratio of expression of Bax/Bcl-2 and expression of apoptotic proteins, viz, p53, PUMA, cleaved caspase-3, and cleaved caspase-9, by referring each gene to GAPDH which was taken as internal control. Values expressed as mean ± SE of three independent blots (*p < 0.05, **p < 0.01, ***p < 0.001)