Fig. 6.

D6-treatment abolishes APP-CTF/SorLA interaction and leads to SorLA-CTFα accumulation in the same intracellular compartments than APP-CTFs. a HEK-293 cells stably expressing C99 were transiently transfected with myc-tagged SorlA and treated or not with D6. SorLA was co-immunoprecipitated with α-APP-CTF antibodies only in vehicle treated cells (upper blot). The middle blot corresponds to the lower part of the immunoblot at higher exposure and reveals the presence of SorLA-CTFα in D6-treated cells. The lower blot corresponds to the same protein samples, but blotted with α-APPct, thus showing the levels of immunoprecipitated APP-CTFs. b Immunoblot analysis of SorLAmyc in cell lysates of vehicle- or D6-treated HEK-C99 cells revealed the presence of SorLA-CTFα-myc in D6-treated cells. c Immunocytochemistry of C99 (red) and SorLAmyc (green) in D6-treated co-transfected HeLA cells