Table 1.
Genetic diversity by country and year
| DRC (2016 and 2017) | DRC (2016) | DRC (2017) | Zambia (2016 and 2017) | Zambia (2016) | Zambia (2017) | Overall | |
|---|---|---|---|---|---|---|---|
| Pfama1 (454 bp) | |||||||
| # Sequences | 301 | 131 | 170 | 255 | 78 | 176 | 554 |
| # Segregating sites (S) | 38 | 38 | 34 | 38 | 38 | 37 | 38 |
| Nucleotide diversity (π) | 0.024 | 0.025 | 0.024 | 0.024 | 0.024 | 0.024 | 0.024 |
| Nucleotide diversity (π): non-synonymous sites | 0.029 | 0.030 | 0.029 | 0.029 | 0.030 | 0.030 | 0.029 |
| # Unique haplotypes | 82 | 53 | 62 | 87 | 46 | 72 | 117 |
| Haplotype diversity (Hd) | 0.975 | 0.976 | 0.974 | 0.978 | 0.978 | 0.978 | 0.977 |
| Average # nucleotide differences (K) | 10.995 | 11.135 | 10.916 | 10.951 | 10.755 | 11.044 | 10.977 |
| Pfcsp (253 bp) | |||||||
| # Sequences | 303 | 106 | 197 | 288 | 66 | 222 | 592 |
| # Segregating sites (S) | 33 | 29 | 32 | 30 | 25 | 30 | 35 |
| Nucleotide diversity (π) | 0.024 | 0.024 | 0.024 | 0.025 | 0.025 | 0.026 | 0.025 |
| Nucleotide diversity (π): non-synonymous sites | 0.030 | 0.030 | 0.030 | 0.032 | 0.031 | 0.032 | 0.031 |
| # Unique haplotypes | 62 | 43 | 55 | 62 | 31 | 56 | 83 |
| Haplotype diversity (Hd) | 0.966 | 0.967 | 0.967 | 0.966 | 0.961 | 0.968 | 0.966 |
| Average # nucleotide differences (K) | 6.052 | 6.101 | 6.033 | 6.418 | 6.231 | 6.483 | 6.214 |
DnaSP (version 6.10.01) was used to calculate the number of segregating sites (S), nucleotide diversity (π) among all loci, nucleotide diversity (π) among non-synonymous sites, the number of unique DNA haplotypes, haplotype diversity (Hd), and the average number of nucleotide differences between P. falciparum haplotype for each of the populations