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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Sep 7;136:42–52. doi: 10.1016/j.yjmcc.2019.09.002

Figure 2: Construction of expression plasmids.

Figure 2:

HcTnI-C27 and HcTnI-C27-H peptides were expressed in Tx3-SUMO-fusion proteins. (A) The amino acid sequences of HcTnI-C27 and HcTnI-C27-H peptides, the Tx3-SUMO fusion protein structure and the strategy of using AgeI cloning site at the fusion joint for the recovery of free peptide with zero fusion residue are illustrated. The N-terminal Tx3 tag in the fusion protein for metal affinity purification is recognized by an mAb 3C11 for immunological identification. (B) The two-step PCR procedure to construct an HcTnI-C27-H mutant cDNA into Tx3-SUMO vector is outlined.