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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Sep 7;136:42–52. doi: 10.1016/j.yjmcc.2019.09.002

Figure 3: Expression and purification of Tx3-SUMO-HcTnI-C27 and Tx3-SUMO-HcTnI-C27-H fusion proteins.

Figure 3:

(A) The SDS-gel shows an example of induced expression of Tx3- SUMO-HcTnI-C27 fusion protein in E. coli and effective one-step Zn(II) column purification. (B) The Western blots of purified fusion proteins showed that while mAb 3C11 bound to both Tx3-SUMO-HcTnI-C27 and Tx3-SUMO-HcTnI-C27-H via the metal binding tag in the fusion carrier, mAb TnI-1 has a strong binding to Tx3-SUMO-HcTnI-C27 indicating preserved epitope structure, which is abolished in the Tx3-SUMO-HcTnI-C27-H mutant. MW, molecular weight.