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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Sep 7;136:42–52. doi: 10.1016/j.yjmcc.2019.09.002

Figure 5: ELISA titration of mAbs TnI-1 and 3C11 against Tx3-SUMO-HcTnI-C27 and Tx3-SUMO-HcTnI-C27-H fusion proteins.

Figure 5:

The fusion proteins were coated on microtiter plate to incubate with serial dilutions of the mAbs for ELISA titration as described in the methods. (A) mAb TnI-1 showed high affinity binding to Tx3-SUMO-HcTnI-C27, which was significantly decreased but still clearly detectable for Tx3-SUMO-HcTnI-C27-H. (B) mAb 3C11 titration curves against the metal tag in fusion proteins confirmed comparable amounts of Tx3-SUMO HcTnI-C27 and Tx3-SUMO-HcTnI-C27-H coated on the microtiter plate. *P < 0.0001 in paired Student’s t-test.