Fig. 4.

Recovery study for different NcLPMO9C concentrations at pH 6.0. Dark gray diamonds represent the found NcLPMO9C concentration (Y-axis) plotted against the added NcLPMO9C concentration (X-axis). The activity was determined at 30 °C using 500 µM hydrocoerulignone and 100 µM H₂O₂ in 100 mM sodium acetate buffer, pH 6.0. The found NcLPMO9C concentration was calculated from the measured volumetric activity using a specific activity of 138 ± 12 U g⁻¹ and the molecular mass of NcLPMO9C (34,400 g mol⁻¹). Gray dashed dotted trace shows the concentration range of NcLPMO9C with the highest accuracy taking the standard deviation into account. The inset shows the low concentration range with the highest precision. All data are expressed as mean values (± SD), from four independent repeats