Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 5;13:63. doi: 10.1186/s40246-019-0238-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — EFTUD2 gene knockdown in HCO cells. a Expression of EFTUD2 mRNA in HCO cells transfected with shNT, sh2, and sh3 lentiviruses. b Protein expression of EFTUD2 in HCO cells transfected with shNT, sh2, and sh3 lentiviruses. c Comparison of cell proliferation between HCO cells with EFTUD2 knockdown (sh2 and sh3) and the control group (shNT). d, e ALP mRNA levels in different groups (shNT, sh2, sh3) at 3 days before and 2 weeks after cell confluence in HCO cells. f Intracellular ALP activity (mu/ml) in HCO cells with EFTUD2 knockdown (sh2 and sh3) was much lower than in the control (shNT) at 3 days before cell confluence according to Student’s t test. g, h COL1A1 mRNA levels in HCO cells in different groups (shNT, sh2, sh3) at 3 days before and 2 weeks after cell confluence. i, j OPN mRNA levels in different groups (shNT, sh2, sh3) of HCO cells during development. k Alizarin red staining of HCO cells in these 3 groups at 3 days after cell confluence. Black arrows indicate calcific nodules (*P < 0.05; **P < 0.01; and ***P < 0.001; Student’s t test)