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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Sci Immunol. 2019 Aug 23;4(38):eaax8704. doi: 10.1126/sciimmunol.aax8704

Figure 1. Immune injury leads the gradual replenishment of the epidermis with LC-like cells.

Figure 1.

A. Male recipients received female BM alone (BMT) or with CD4 and CD8 (Matahari) T cells. Chimerism was measured within the mature CD11b+Langerin+ LC population at different time points. Representative flow plots show the relative frequency of host (CD45.2) and donor (CD45.1)-derived cells. B. Graph showing the frequency ± SD of donor LC in mice receiving BMT with (circles) or without (triangles) T cells. Significance was determined with a 2-way ANOVA, ***P<0.001. Data are pooled from 2 independent experiments for each time point (n=5–10). C. Graph shows the number ± SD of Vβ8.3+ Matahari (Mh) T cells in the epidermis over time, per 0.1g total ear tissue (n=7–8). D. Top - representative histogram overlays show the expression of LC-associated proteins on donor-derived LC (from mice that received BMT + T cells) or host eLC (BMT alone), 10 weeks post-transplant. Bottom - summary data showing the median fluorescent intensity (MFI) for each sample. H = host, D = donor, each symbol is one mouse. Data are pooled from 2 independent experiments (n=8), and representative of >3 different experiments.