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. 2019 Nov 13;6(11):190773. doi: 10.1098/rsos.190773

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© 2019 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Characterization of strains bearing mutations in ureA codons 24 and 25. Growth phenotypes of mutant UreA strains at 25°C and 37°C on urea as nitrogen source or on 2-thiourea with sodium nitrate (NaNO₃) 10 mM as nitrogen source to test resistance to the analogue. Growths on 5 mM ammonium (NH₄) and 10 mM NaNO₃ are used as controls. A wt ureA::gfp and a ureAΔ strain are shown as positive and negative controls, respectively.