Fig 2. Inhibitory activities of IBP-conjugated peptides against HIV-1 infection and biophysical properties of CHR/NHR peptide complexes.
(A) Inhibitory activities of T20, CP24, IBP, CP24-IBP and IBP-CP24 against infection of HIV-1 IIIB (subtype B, X4). (B) Inhibitory activities of T20, CP24, IBP, CP24-IBP and IBP-CP24 against infection of HIV-1 Bal (subtype B, R5). (C) Inhibition of Env-mediated cell-cell fusion between H9/HIV-1IIIB cells and MT-2 cells. The data are presented as mean ± SD. (D) The secondary structure of the complexes of N36 and C34, CP24, IBP or IBP-CP24 was determined by CD. The circular dichroism spectra of these complexes displayed typical double minima at 208 and 222 nm for the α-helical feature. C34 and IBP were used as positive and negative controls, respectively. (E) Thermostability of the complexes of N36 with C34, CP24 or IBP-CP24 was measured by CD. (F) Inhibitory activities of CP24, IBP and IBP-CP24 against 6-HB formation between N36 and C34. Each sample was tested in triplicate and the data are presented as mean ± SD.