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. 2019 Dec 5;15(12):e1008082. doi: 10.1371/journal.ppat.1008082

Fig 3. In vitro human and rhesus monkey IgG binding and in vivo pharmacokinetic studies of CP24 and IBP-CP24.

Fig 3

(A) Binding affinity of CP24, IBP and IBP-CP24 to human IgG was determined by ELISA. (B) Binding affinity of CP24, IBP and IBP-CP24 to rhesus monkey IgG was measured by ELISA. Each sample was tested in triplicate and the data are presented as mean ± SD. (C) Binding affinity of CP24, IBP and IBP-CP24 to rhesus monkey IgG was evaluated by isothermal titration calorimetry (ITC) assay. The top panel showed the titration traces inhibitors solution injected into rhesus monkey IgG solution. The bottom panel showed the binding affinity of inhibitors to rhesus monkey IgG. (D) Plasma concentrations of CP24 and IBP-CP24 in vivo. Rhesus monkeys were administered intravenously with a single dose of 10 mg/kg CP24 (n = 2) or IBP-CP24 (n = 3). The concentrations were determined by sandwich ELISA. Each sample was tested in triplicate and data were presented as mean ± SD.