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. 2019 Dec 5;14(12):e0225911. doi: 10.1371/journal.pone.0225911

Fig 3. SMAD2/3 inhibition attenuates HG-induced DDR2 and collagen expression.

Fig 3

Sub-confluent quiescent cultures of vascular adventitial fibroblasts in M199 were pre-treated with SMAD2 inhibitor, PD169316, and SMAD3 inhibitor, SIS3, for 1 h and subsequently with HG for 12 h. Post-HG treatment, protein was isolated and subjected to western blot analysis for detection of: (A and B) Collagen type 1 alpha 1; *p< 0.05 vs. control, ##p< 0.01 vs. HG (One-way ANOVA p< 0.05) and (C and D) DDR2; *p< 0.05 vs. control, ##p< 0.01 vs. HG. (One-way ANOVA p< 0.05). β-actin was used as loading control. Data are representative of three independent experiments, n = 3. Error bars represent SD.