Skip to main content
. 2019 Dec 5;14(12):e0225740. doi: 10.1371/journal.pone.0225740

Fig 4. Combination of BLM inhibitor ML216 and DSBs equidistant to the HLA class III and I genes in the short arm of chromosome 6 results in homozygosity of HLA class I.

Fig 4

(A) Procedure for the isolation of HLA-homozygous clones from hiPSC-telHLAcNP/+ cells under the condition of BLM protein suppression. (B) Schematic representation of the short arm of chromosome 6. After targeting the double selection cassette (telHLAcNP/+), an allele-specific DSB was introduced between HLA class I and III genes. The HLA-heterozygous genotypes in parental cells are shown in the right panel. (C) Schematic representation of the short arm of chromosome 6 after crossover. Patterns of heterozygous and LOH genotypes are shown in the left panel. HLA genotypes after crossover in clone 1 are shown in the right panel. (D) SNP array analysis of hiPSC-telHLAcNP/+ML216(+)HLA I-III-CRISPR(+). (E) Flow cytometry profile of the surface expression of the HLA-A haplotype A2 and A32 in hiPSCs.