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. 2019 Dec 5;14(12):e0225598. doi: 10.1371/journal.pone.0225598

Fig 1. Osteogenic medium (OM) induces the expression of osteogenic markers at mRNA and protein levels in SHED cells.

Fig 1

A and B: Phase-contrast micrographs of SHED cells at 7 days in the presence of basal (A) or osteogenic medium. Alkaline phosphatase (ALP) stained cells are shown in Panel B. Magnification is 100X. C-E: SHED cells treated with basal (-) or osteogenic medium (OM; (+)). RT-PCR (C) and immunoblotting (D) of indicated osteogenic markers to determine mRNA and protein levels, respectively. GAPDH was used as a loading control in C and D. E. Expression of OPN, RUNX2, and Osterix proteins were quantitated from three different experiments, and the expression levels are provided as the percent increase for each protein. Error bars represents SD. **** p< 0.001; **p<0.01 vs. basal medium (-).