Fig 1. scRNA-seq of mouse and NM-R spleens reveals major differences in immune cell populations.

UMAP projections of four mouse (A) and four NM-R (B) spleens. Each point is a cell color-coded by its cluster assignment and annotated cell type (Materials and methods). The proportions (%) of cells assigned to each cell type in each species (excluding NM-R clusters of erythroid cells) are shown in the stacked bar charts. Gene-by-cell expression-level heatmaps in the mouse (C) and NM-R (D) spleens. Selected marker genes are listed to the left and cells are faceted by their cluster assignment. UMAP projections of the NM-R spleens color-coded by the expression levels of Nkg7 and Gzma (E), Cd3e and Cd8a (F), and Cpa3 (G). Gene-by-cell expression-level heatmap of the mouse spleen T cells and NK cell clusters and NM-R spleen T cells and mast cell clusters (H). APC, antigen-presenting cell; DC, dendritic cell; NK, natural killer; NKT, natural killer T; NM-R, naked mole-rat; RP, red pulp; scRNA-seq, single-cell RNA-sequencing; UMAP, uniform manifold approximation and projection; UMI, unique molecular identifier.