Static, normal, volume overload, and pressure overload samples labelled ‘static’, ‘normal’, ‘pressure’, and ‘volume’ respectively (N=5 for each group). The volume overload showed a significant increase in length and is clearly elongated in the image so much so that it has buckled once it returned to its original resting state. The increase in length resulted in a significant decrease in aspect ratio compared to static, normal, and pressure overload samples. Pressure overload samples significantly increased in width but not length, resulting in a significant increase in aspect ratio when compared to static, normal, and volume overload samples. The difference between normal and static samples was not significant although a decrease in aspect ratio was found in the normal samples. (Scale = 4 mm)