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(A) Laurdan fluorescence of 100% POPC nanodiscs acquired at 5°C (red), 15°C (yellow), 25°C (green), 35°C (cyan), and 45°C (purple). A redshift in the spectra is observed at increasing temperatures (denoted by arrows). (B) Relative laurdan fluorescence of nanodiscs containing 100% DMPC (red), 80% DMPC/20% POPC (yellow), 50% DMPC/50% POPC (green), 20% DMPC/80% POPC (cyan), or 100% POPC (purple). A redshift in the spectra is observed with increasing POPC content (denoted by arrows).
