Skip to main content
. 2019 Dec 5;8:e50583. doi: 10.7554/eLife.50583

Figure 7. LIN-44/Wnt-dependent localization of LIN-17/Fz at the PDB posterior neurites.

(A–C) Representative images of LIN-17::GFP localization (top panels), PDB neurite structure labeled with cytoplasmic BFP (middle panels) and merged images (bottom panels) in wildtype (A), lin-44(n1792) (B), and nrt-bfp-lin-44(miz56) (C) animals, respectively. Magnified images of the tip of anterior and posterior neurites are shown in the right panels. (D) Quantification of the normalized GFP/BFP signal ratio at the anterior and posterior growth cones. Error bars indicate mean ± SEM. ***p<0.001; n.s., not significant (Ratio paired t-test).

Figure 7—source data 1. Quantification of the normalized GFP/BFP signal ratio at the anterior and posterior growth cones.

Figure 7.

Figure 7—figure supplement 1. Wnt-dependent localization of DSH-1/Dsh at the PDB posterior neurites.

Figure 7—figure supplement 1.

(A–D) Representative images of DSH-1::GFP localization (top panels), PDB neurite structure labeled with cytoplasmic BFP (middle panels) and merged images (bottom panels) in wildtype (A), lin-44(n1792) (B), lin-17(n671) (C) and nrt-bfp-lin-44(miz56) (D) animals, respectively. Magnified images of the tip of anterior and posterior neurites are shown in the right panels. (E) Quantification of the normalized GFP/BFP signal ratio at the anterior and posterior growth cones. Error bars indicate mean ± SEM. ***p<0.001; n.s., not significant (Ratio paired t-test).
Figure 7—figure supplement 1—source data 1. Quantification of the normalized GFP/BFP signal ratio at the anterior and posterior growth cones.