cam-1(gm122): |
|
Forward: |
aacttacactctgaagcgccgtgc |
Reverse: |
ccaacttcagatgatggatcg |
egl-20(n585): |
wildtype PCR product can be digested with HpyCH4V: |
Forward: |
cttacctctcaaatttgaacttattcttgc |
Reverse: |
cctcattaccattcaactgatag |
fmi-1(tm306): |
|
Forward: |
gtgaaattacatgttaactgagg |
Reverse: |
tcgcctacaagaagtaacttacatg |
lin-44(n1792): |
wildtype PCR product can be digested with NcoI: |
Forward: |
gtgcgaatcgtttgagatttcagccatg |
Reverse: |
catctggttgttacacgcacaatcg |
mig-1(e1787): |
mutant PCR product can be digested with BgIII: |
Forward: |
acagcacaaaattcaaagccctc |
Reverse: |
atttttgagccattcaaaaagaattttgaagatc |
sax-7(nj48): |
|
Forward: |
tgagatgaaagaaggaggagtgc |
Reverse: |
cacacacaatggcgcacaag |
lin-17(n671): |
wildtype PCR product can be digested with MfeI: |
Forward: |
ccgcatttttcgtagatcacaccg |
Reverse: |
actgttgtttacagtcaattgtcattcgggtcaatt |
vang-1(ok1142): |
|
Forward: |
accttgacaacgccagacag |
Mutant reverse: |
gcggcaattaggcgatacctg |
Wildtype reverse: |
cgagcgaagtttgagtgaatc |
dsh-1(ok1445): |
|
Forward: |
cttgagatagccctgcaagac |
Reverse: |
gctccaccacttgctaagattg |
mig-5(tm2639): |
|
Forward: |
aagcattcgctcctcatcatc |
Reverse: |
atctcgacgatgaaacgactc |
nrt-bfp-lin-44(miz56): |
|
Wildtype forward: |
ggaaatagtgtgtggatgag |
Mutant forward: |
aaggaaccgtggacaaccatc |
Reverse: |
ccactgatcgtcgggatctc |
sdn-1(ok449): |
|
Forward: |
agaagtgtctcgttcagtgg |
Wildtype reverse: |
ctgcaaagccgacaacggtac |
Mutant reverse: |
gagatgccggtcaggtgattac |
cwn-1(ok546): |
|
Forward: |
agctggctaggtcccagaag |
Reverse: |
tcgaacgatcctcttcagtac |