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. 2019 Nov 19;8:e48178. doi: 10.7554/eLife.48178

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© 2019, Peng et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Appendix 1—figure 24. — The following steps connect the precision pressure regulators (x, 10–1000 mbar) to the valve manifold to generate HIGH pressure (70 mbar, green lines) and LOW pressure (20 mbar, blue lines).

54. Connect 2 PE-tubes to the multiple distributor with four outlets and each to one of the precision regulators. Pay spetial attention to intended direction of airflow on the regulators. Arrowheads indicate direction of air flow.

55. Connect the HIGH pressure regulator (set to 70 mbar) to the middle nozzle of the single 3/2 solenoid valve which is attached to the acrylic plate (green line). Connect the PE-tubing with a 2/4 mm silicone tube using a mini tubing connector(*).

56. Connect the LOW pressure regulator (set to 20mbar) to the 1P nozzle of the top level 3/2 valve manifold. Use a 4/6 mm silicone tube to connect the PE-tubing with the nozzle.