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. 2019 Nov 19;8:e48178. doi: 10.7554/eLife.48178

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© 2019, Peng et al

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Appendix 1—figure 26. — 59. Plug in the power supply (12 V / 6.67 A, 80 W) and the USB-cable to the Arduino board.

60. Connect to presurrised air supply using a PE-tubing with 8 mm outer diameter. The air supply should be set at sufficient high pressures (e.g. 5 bar).

Adjust the regulators manually to the desired pressures.

*The pressure regulator upstream of the vacuum ejector needs to be adjusted while measuring the negative pressure generated by the vacuum ejector. It should be set at a pressure at which −350 mbar can be applied to the valve manifold (approx. 3–5 bar). To control the device, you can use the Matlab code and GUI we provided. One could also control the Arduino board using any custom script. Before the first use, please transfer the mapping of the individual valves onto their relay number and respective Arduino digital output pin into the script (see guide to Matlab GUI). Now the pressure control device is fully operational.