Figure 5. Different strategies for cleaning pipettes.
(A) Scheme of successful whole-cell recordings on six pipettes (blue circles with action potentials) and failed patch attempts on two pipettes (white circles without action potentials). (B) Clean-to-complete: The two failed pipettes were cleaned and successful recordings were established from two neighbouring cells. Red circles represent a cluster that has been subject to pipette cleaning after failed patch attempts and the red lines indicate the tested synaptic connections. (C) Dot plot comparing the number of successfully recorded neurons in clusters without cleaning (blue) and with cleaning (red). Black crosses indicate mean and standard deviation. See Figure 5—source data 1. (D) Clean-to-extend: After recording of a cluster, individual pipettes can be cleaned and used to patch additional cells (yellow circles). Yellow lines indicate the synaptic connections that could be tested due to this approach. (E) Stacked bar graph depicting the number of tested connections of the initially recorded cluster with clean-to-complete (red) and the number of additionally tested connections after clean-to-extend (yellow) from five animals. Data were extracted from experiments in the rat presubiculum on the 8-manipulator setup. Two to three slices were analyzed from each animal in a time window of 5 to 6 hr. Intersomatic distance distribution of these experiments are shown in the figure supplement to this figure. Source data provided in Figure 5—source datas 1 and 2.
Figure 5—figure supplement 1. Intersomatic distance distribution Box plots depicting the intersomatic distances of tested connections from rat presubiculum experiments in different clean2extend recording rounds.
