Figure 5: Arp2/3 inhibition increases brush border F-actin levels at the expense of basolateral cortical actin networks.
(A) Confocal images of phalloidin-stained mock and CK-666-treated W4 cells after 3 hours in doxycycline. (B) Distribution of phalloidin intensities in mock and CK-666-treated W4 cells, measured from ROIs drawn to surround the basolateral margin (i.e. cortex) of the cell; number of data points is given under each boxplot. ANOVA followed by Tukey post hoc test, **p < 0.0025. (C) Distribution of phalloidin intensities in mock and CK-666-treated W4 cells, measured from ROIs drawn to surround the brush border (BB); number of data points is given under each boxplot. ANOVA followed by Tukey post hoc test, **p < 0.0001.