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. 2019 Nov 29;10:2783. doi: 10.3389/fmicb.2019.02783

FIGURE 1.

FIGURE 1

T4 phages sensitivity test for E. coli strains MG1655 and EcN by (A) phage plaque assay: 10 μl of serially diluted [undiluted (UD) to 10– 7 Pfus/ml] T4 phage lysate was spotted on either MG1655 or EcN lawn in 0.7% LB agar and incubated at 37°C for 24 h, static. (B) Liquid culture assay: 100 μl of 109 Pfus/ml T4 phage lysate was added to mid-log growing phase EcN or MG1655 culture (OD600∼0.5) and incubated at 37°C for 4 h, 180 rpm. Imaging was performed with Canon PowerShot SX260 HS and processed with ImageJ 1.50i.