FIGURE 7.

T4 phage inactivation by differentially treated EcN samples: Graph (A,B) illustrate the results of coincubation studies performed under various conditions as described: processed EcN samples were further treated with 25 μg/ml polymyxin B (PMB) for 1 h at 37°C and used for the coincubation studies. Pfus/ml were determined after 24 h at 37°C using standard phage plaque assay (A). Legends of the graph are described in Figure 5C. The heat-killed EcN cells were further treated with increasing concentration of PMB: 1× – 25 μg/ml, 5× – 125 μg/ml, 10× – 250 μg/ml. As a control, LB medium was treated with the same concentrations of PMB and used in the incubation. Pfus/ml were determined after 24 h at 37°C using standard phage plaque assay. The asterisks depict the statistical significance of the different samples when compared to the control with LB medium or water. For the samples treated with PMB, the statistical significance was analyzed in comparison to the respective sample without PMB. ns – not significant, ^∗∗p < 0.01, ^∗∗∗p < 0.001 and ^∗∗∗∗p < 0.0001.