TABLE 3.

PCR conditions for localization of T4 phage DNA by T4 specific PCR.

PCR reaction mix	1×	PCR condition
2× PCR MM	12.5 μl	Step 1: 95°C for 5 min
T4_ndd_F	1.25 μl	Step 2: 95°C for 30 s
T4_ndd_R	1.25 μl	Step 3: 55°C for 1 min
Plasmid pKD3	1 μl (30 ng)	Step 4: 72°C for 2 min
pKD3_F	1.25 μl	Step 2 to 4–35 cycles
pKD3_F	1.25 μl	Step 5: 72°C for 10 min
Template	2 μl	Step 6: hold at 4°C
Water	Upto 25 μl