Table 1.
Exudate volume, PMN cell number, MPO activity in lung tissue, and TNF-α, IL-1β, IL-6 and IL-10 levels in BALF from the V + nonheated, V + heated (42 °C for 80 min), V + OT (20 µg/kg) + heated and L-368,899 + OT (20 µg/kg) + heated groups.
| Parameters | V + nonheated | V + heated | V + OT + heated | L-368,899 + OT + heated |
|---|---|---|---|---|
| 1. Exudate volume (mL) | 0.3 ± 0.1 | 3.2 ± 0.2b | 1.5 ± 0.2e | 3.4 ± 0.3d |
| 2. PMN cells (million cells/rat) | 15 ± 6 | 174 ± 8b | 82 ± 6e | 181 ± 9d |
| 3. MPO activity (μg/mg protein) | 97 ± 14 | 326 ± 17b | 133 ± 19e | 329 ± 18d |
| 4. TNF-α (pg/mL) | 9 ± 2 | 69 ± 4b | 22 ± 5e | 72 ± 5d |
| 5. IL-1β (pg/mL) | 18 ± 3 | 77 ± 5b | 24 ± 4e | 80 ± 6d |
| 6. IL-6 (pg/mL) | 12 ± 1 | 78 ± 3b | 26 ± 3e | 79 ± 4d |
| 7. IL-10 (pg/mL) | 31 ± 5 | 57 ± 6b | 123 ± 8e | 53 ± 5d |
Data are presented as the mean and SD for ten rats/group. bP < 0.05 compared with the V + nonheated group; eP < 0.05 compared with the V + heated group; dP < 0.05 compared with the V + OT + heated group.
Rats were sacrificed 100 min after the initiation of heat stress by an overdose of anesthetic, and the samples were collected for biochemical determination.