Fig. 3. Lack of acp3U47 results in genome instability in E. coli.
a Morphological alteration of WT (left) or ΔtapT (right) strains after cultivation at 42 °C for 4 days. Small colonies appeared in the ΔtapT strain. b Frequencies of small colony appearance of ΔtapT strain with continuous cultivation at 37 °C (closed circles) and 42 °C (open circles). Data represent average values of biological triplicates, with s.d. Source data are provided as a Source Data file. c Frequencies of small colonies in WT, ΔtapT, and ΔtapT overexpressing tapT after cultivation at 42 °C for 4 days. Data represent average values of biological triplicates, with s.d. Source data are provided as a Source Data file. d Motility test of the normal colony-derived cells (left), small colony-derived cells (middle), and small colony-derived cells overexpressing tapT (right). Diameter of halo was defined as the length of the line segment which passes through the center and the ends of the halo. Data represent average values of biological replicates (n = 5), with s.d. Source data are provided as a Source Data file. e Morphology of the small colony-derived cells (left) and the small colony-derived cells overexpressing tapT (right). Overnight saturated cultures were diluted, spotted onto LB plates, and cultivated at 37 °C.